Publications
Our group is dedicated to advancing the field of chemical biology and drug discovery through cutting-edge research. Our work focuses on understanding the complex cellular processes involved in cell signaling and the myriad of molecular interactions that drive them.
2023
Singaram, I., Sharma, A., Pant, S., Lihan, M., Park, M.-J., Pergande, M., Buwaneka, P., Hu, Y. Mahmud, N. Kim, Y.-M. Cologna, S. Gevorgyan, V. Khan, I. Tajkhorshid, E. and Cho, W. Targeting lipid-protein interaction to treat Syk-mediated acute myeloid leukemia (2023) Nature Chemical Biology, 19, 230-250 DOI: 10.1038/s41589-022-01150-z/NCHEMB-A210913789 (Highlighted in Nature Reviews in Drug Discovery and Cancer Discovery)
Cho W, Berkley K, Sharma A. Lipid Binding of SH2 Domains. Methods Mol Biol. 2023;2705:239-253. doi: 10.1007/978-1-0716-3393-9_13.PMID: 37668978
Ning D, Wang ZG, Wang L, Tian YF, Jing F, Jiang LH, Zhang MQ, Liu YY, Pang DW, Cho W, Liu SL., Lipid-Centric Design of Plasma Membrane-Mimicking Nanocarriers for Targeted Chemotherapeutic Delivery. Advanced Materials. 2023 Sep 21:e2306808. doi: 10.1002/adma.202306808. PMID: 37732588
Jiachen Sun, Seohyeon Song, Indira Singaram, Yusi Hu, (Jean Zhao) Volker Haucke, and Wonhwa Cho. PI(3,5)P2 Controls the Signaling Activity of Class I PI3K: Its Implication in Cancer. bioRxiv. doi: 10.1101/2023.01.25.525550.
Rizalar FS, Lucht MT, Petzoldt A, Kong S, Sun J, Vines JH, Telugu NS, Diecke S, Kaas T, Bullmann T, Schmied C, Löwe D, King JS, Cho W, Hallermann S, Puchkov D, Sigrist SJ, Haucke V. Phosphatidylinositol 3,5-bisphosphate facilitates axonal vesicle transport and presynapse assembly. Science. 2023 Oct 13;382(6667):223-230. doi: 10.1126/science.adg1075. Epub 2023 Oct 12.PMID: 37824668
2022
Liqian Ma, Wonhwa Cho, and Erik R. Nelson Our evolving understanding of how 27-hydroxycholesterol influences Cancer, (2022) Biochemical Pharmacology, 196:114621. doi: 10.1016/j.bcp.2021.114621
Wonhwa Cho, Arthur Ralko, Ashutosh Sharma, and Shu-Lin Liu An In situ fluorescence assay for cholesterol transport-like activity of the Patched. (2022) Methods in Molecular Biology 2374, 37-47.
Ha Pham, Indira Singaram, Jiachen Sun, Arthur Ralko, Madalyn Puckett, Ashutosh Sharma, Alice Vrielink, and Wonhwa Cho, Development of a novel spatiotemporal depletion system for cellular cholesterol (2022) J Lipid Res, 63, 100178. doi: 10.1016/j.jlr.2022.100178
2021
Buwaneka, P., Ralko, A., Gorai, S., Pham, H. & Cho, W. Phosphoinositide-binding activity of Smad2 is essential for its function in TGF-β signaling. Journal of Biological Chemistry 297
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Yang, Fan, et al. The Impact of TiO2 Nanoparticle Exposure on Transmembrane Cholesterol Transport and Enhanced Bacterial Infectivity in HeLa Cells. Acta Biomaterialia., vol. 135, Elsevier, 2021, pp. 606–16
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Ma, L., Cho, W. & Nelson, E. R. Our evolving understanding of how 27-hydroxycholesterol influences cancer. Biochem Pharmacol 114621
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Buwaneka, P., Ralko, A., Liu, S.-L. & Cho, W. Evaluation of the available cholesterol concentration in the inner leaflet of the plasma membrane of mammalian cells. J Lipid Res 62, 100084
2020
Zhang, Y. et al. Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit. Proc Natl Acad Sci U S A 117, 28838–28846 (2020).
Koponen, A. et al. ORP2, a cholesterol transporter, regulates angiogenic signaling in endothelial cells. FASEB J 34, 14671–14694 (2020).
Sharma, A. et al. Photostable and Orthogonal Solvatochromic Fluorophores for Simultaneous In Situ Quantification of Multiple Cellular Signaling Molecules. ACS Chem. Biol. 15, 1913–1920 (2020).
Sun, J., Singaram, I., Soflaee, M. H. & Cho, W. A direct fluorometric activity assay for lipid kinases and phosphatases. Journal of Lipid Research 61, 945–952 (2020).
2019
Bae, D.-J. et al. ArhGAP12 plays dual roles in Stabilin-2 mediated efferocytosis: Regulates Rac1 basal activity and spatiotemporally turns off the Rac1 to orchestrate phagosome maturation. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research 1866, 1595–1607 (2019).
2018
Kim E, Kim DH, Singaram I, Jeong H, Koh A, Lee J, Cho W*, and Ryu SH*. Cellular phosphatase activity of C1-Ten/Tensin2 is controlled by Phosphatidylinositol-3,4,5-triphosphate binding through the C1-Ten/Tensin2 SH2 domain (2018) Cell Signal. 51, 130-138 (*Co-corresponding authors)
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Liu, S.-L., Wang, Z., Hu, Y., Xin, Y., Gorai, S., Sharma, A., Zhou, X., Gong, L-W., Hay, N., Zhang, J. and Cho, W. Quantitative lipid imaging reveals a new signaling function of phosphatidylinositol-3,4-bisphosphate: Isoform and subcellular site-specific activation of Akt (2018) Molecular Cell 71, 1092-1104 doi: 10.1016/j.molcel.2018.07.035
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Zhang, Y., Bulkley, D.P., Xin, Y., Roberts, K.J., Asarnow, D.E., Sharma, A., Myers, B.R., Cho, W.*, Cheng, Y.*, Beachy*, P.A. Structural basis for cholesterol transport-like activity of the Hedgehog receptor Patched (2018) Cell 175, 1352-1364 (*Co-corresponding authors)
2017
Liu, S.-L., Sheng, R., Jung, J.H., Wang, L., Stec, E., O’Connor, M.J. Song, S., Bikkavilli, R.K., Baek, K., Winn, R.A., Lee, D., Ueda, K., Levitan, I., Kim, K.-P., and Cho, W. (2017) Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol Nature Chemical Biology, 13, 268-274
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Cho, W., Hu, Y., Baek, K., Kim, H. (2017) A High-throughput fluorometric assay for lipid–protein binding Methods Enzymol. 583, 1-18
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Cho, W., Yoon, Y., Liu, S.-L., Baek, K., Sheng, R. (2017) Fluorescence-based in situ quantitative imaging for cellular lipids Methods Enzymol. 583, 19-33
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Ahn, G., Kim, H., Kim, D., Hanh, H., Yoon, Y., Zhuang, X., Liang, Z., Adu-Gyamfi, E., Stahelin, R.V., Jiang, L., Cho, W., Kang, B.-H., Hwang, I. (2017) SH3P2 as a key factor in membrane structure modulation during cell plate formation in plants Plant Cell 29, 1388-1405
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Wood, M. N., Ishiyama, N., Singaram, I., Chung, C., Flozak, A.S., Yemelyanov, A., Ikura, M., Cho, W. and Gottardi, C.J. (2017) α-Catenin homodimers are recruited to phosphoinositide-activated membranes to promote adhesion, J. Cell Biol. 216, 3767-3783.
2015
Tyson, G.H., Halavaty, A., Kim, H., Geissler, B., Agard, M., Cho, W., Anderson, W., Hauser, A. (2015) A Novel Phosphatidylinositol 4,5-bisphosphate Binding Domain Mediates Plasma Membrane Localization of ExoU and Other Patatin-like Phospholipases J. Biol. Chem. 290, 2919-2937
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Agarwal, S., Kim, H., Chen, R.B., Agarwal, S., Williamson, R., Cho, W., Di Paolo, G., and Satchell, K. J. F. (2015) Autophagy and endosomal trafficking inhibition by Vibrio cholerae MARTX toxin phosphatidylinositol-3-phosphate specific phospholipase A1 activity Nature Commun 6:8745. doi: 10.1038/ncomms9745.
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2014
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Sheng, R., Kim, H., Lee, H., Xin, Y., Chen, Y., Tian, W., Choi, J.-C., Doh, J., Han, J.-K. and Cho, W. (2014) Cholesterol Selectively Activates Canonical Wnt Signaling over Non-Canonical Wnt Signaling. Nature Commun. 5:4393 doi: 10.1038/ncomms5393
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Kim, D.H., Park, M.-J., Gwon, G.H., Silkov, A., B. Xu, Z.-Y, Yang, E.C., Song, K., Song, K., Kim, Y., Yoon, H.S., Honig, B., Cho, W., Cho, Y., and Hwang, I.* (2014) An Ankyrin Repeat Domain of AKR2 Drives Chloroplast Targeting through Coincident Binding of Two Chloroplast Lipids Dev. Cell 30, 598-609 (*Co-corresponding authors)
Liu, S.-L., Sheng, R., O’Connor, M.J., Cui, Y., Yoon, Y.
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Kurilova, S., Lee, D., and Cho, W. (2014) Simultaneous In Situ Quantification of Two Cellular Lipid Pools Using Orthogonal Fluorescent Sensors Angew. Chem. Int. Ed. 53, 14387-14391
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Bhardwaj, N., Cho, W., and Lu, H. (2014) MetaDoR: Online resource and prediction server for membrane targeting peripheral proteins in Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics, Eds., Pan, Y., Li, M., Wang, J. Wiley Series on Bioinformatics, Wiley.
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2012
BL, Levine AJ, Materi AM, Song WL, Keku TO, Goodman JE, Sansbury LB, Das S, Dai A, Kwong AM, Lin AM, Lin JM, Park JM, Patterson RE, Chlebowski RT, Garavito RM, Inoue T, Cho W, Lawson JA, Kapoor S, Kolonel LN, Le Marchand L, Haile RW, Sandler RS, Lin HJ. (2012) Hematopoietic prostaglandin D synthase (HPGDS): A high stability, Val187Ile isoenzyme common among African Americans and its relationship to risk for colorectal cancer. Prostaglandins Other Lipid Mediat. 97, 22-28. PMCID: PMC3226866
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M. Rowland, D. Gong, H. E. Bostic, N. Lucas, W. Cho, and M. D. Best (2012) Microarray Analysis of Akt PH Domain Binding Employing Synthetic Biotinylated Analogs of All Seven Phosphoinositide Headgroup Isomers Chem. Phys. Lipids 165, 207-215. doi: 10.1016/j.chemphyslip.2011.12.001.
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Chen, Y., Sheng, R., Kallberg, M., Silkov, A., Tun, M.P., Bhardwaj, N., Kurilova, S., Hall, R.A., Honig, B., Lu, H., and Cho, W. (2012) Genome-Wide Functional Annotation of Dual Specificity Protein- and Lipid-Binding Modules That Regulate Protein Interactions Molecular Cell 46, 226-237.PMCID: PMC3431187 [Featured in Faculty of 1000, ScienceDaily (Cell Protein Interactions Favor Fats) and UIC News (Fats are control freaks that want to rule the cells)
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Stahelin, R.V., Kong, K-F., Raha, S., Tian, W., Melowic, H., Murray, D., Altman, A., and Cho W., (2012) Protein kinase Cθ C2 domain is a phosphotyrosine-binding module that plays a key role in its activation. J. Biol. Chem. 287, 30518-30528 PMCID: PMC3436300
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K. Song, M. Jang, S. Y. Kim, Y. J. Lee, W. Cho, and I. Hwang (2012). An A/ENTH domain-containing protein co-localizes with clathrin-coated vesicles on the growing cell plate in Arabidopsis root cells Plant Physiol 159,1013-25
Gil, J.-E., Kim, E., Kim, I.-S., Ku, B., Park, W.S., B.-H. Oh, Ryu, S. H., Cho, W., Heo W.D (2012) Phosphoinositides differentially regulate protrudin localization through the FYVE domain (*corresponding authors) J. Biol. Chem. 287, 41268-41276 PMID: 23043110
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Sheng, R., Chen, Y., Gee, H. Y., Stec, E., Melowic, H.R., Blatner, N.R., Tun, M.P, Kim, Y., Kallberg, M., Fujiwara, T., Hong, J.H., Kim, K.P., Lu, H., Kusumi, A., Lee, M.G.and Cho W. (2012) Cholesterol Modulates Cell Signaling and Protein Networking by Specifically Interacting with PDZ Domain-Containing Scaffolding Proteins Nature Communications 3, 1249 DOI: 10.1038/ncomms2221 [Featured in National Institute of General Medical Sciences (NIH NIGMS) News and UIC News
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2010
Yoon, Y., Tong, J. Lee, P.J., Albanese, A., Bhardwaj, N., Digman, M.A., Lu, H., Gratton, E., Shin, Y.-K. and Cho, W., (2010) Molecular basis of the potent membrane remodeling activity of the epsin1 ENTH domain J. Biol. Chem., 285, 531-540
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Capraro, B. R., Yoon, Y., Cho, W., and Baumgart T. (2010), Curvature sensing by the epsin N-terminal homology (ENTH) domain measured on cylindrical lipid membrane tethers J. Amer. Chem. Soc. 132,1200-1 PMID: 20050657
2008
Tian, W., Wijewickrama, G. T., Kim, J. H., Das, S., Tun, M. P., Gokhale, N., Jung, J.W., Kim, K. P., and Cho, W. “Mechanism of Regulation of Group IVA Phospholipase A2 Activity by Ser727 Phosphorylation,” J. Biol. Chem. 283, 3960-3971
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Das A, Base C, Manna D, Cho W, Dubreuil RR. (2008) “Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton,” J. Biol. Chem. 283, 12643-2653 [PMID: 18287096]
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Manna, D., Bhardwaj, N., Vora, M.S., Stahelin, R.V., Lu, H., and Cho, W., (2008) Relative Importance of Phosphatidylserine and PtdIns(4,5)P2 in Plasma Membrane Targeting of Cellular Proteins: Molecular Dynamics Simulation and Membrane Binding Studies of the PKCα C2 Domain J. Biol. Chem. 283, 26047–26058.
2004
Stahelin, R.V. Digman, M.A., Medkova, M. Ananthanarayanan, B., Rafter, J.D., Melowic, H. and Cho, W. (2004) “Mechanisms of diacylglycerol-induced membrane binding and activation of protein kinase Cd” J. Biol. Chem. 279, 29501-29512.
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Blatner, N.R., Stahelin, R.V., Diraviyam, K. Bruzik, K.S., Hawkins, P.T., Hong, W., Murray, D., and Cho, W. (2004) “The Molecular Basis of the Differential Subcellular Localization of FYVE Domains,” J. Biol. Chem. 279, 53818-53827
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Stahelin, R.V., Ananthanarayanan, B., Blatner, N.R., Singh, S., Bruzik, K.S. Murray, D., and Cho, W. (2004) “Mechanism of Membrane Binding of the Phospholipase D1 PX Domain,” J. Biol. Chem. 279, 54918-54926.
2002
Ananthanarayanan, B., Das, S., Rhee, S.G., Murray, D. and Cho, W. (2002) “ Membrane Targeting of C2 Domains of Phospholipase C-d Isoforms” J. Biol. Chem. 277, 3568-3575.
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Beers, S.A., Buckland, A.G., Koduri, R.S. Cho, W., Gelb, M.H., and Wilton, D.C. (2002) “The antibacterial properties of secreted phospholipase A2: A major physiological role for the IIA enzyme that depends on the very high pI of the enzyme to allow penetration of the bacterial cell wall” J. Biol. Chem. 277, 1788-1793
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Han, J.M., Kim, J.H., Lee, B.D., Lee, S.D., Kim, Y., Chung, Y.W., Lee, S., Cho, W., Ohba, M., Kuroki, T., Suh, P.-G. and Ryu, S.H. (2002) “Phosphorylation-dependent regulation of Phospholipase D2 by Protein Kinase C in Rat pheochromocytoma PC12 cells,” J. Biol. Chem. 277, 8290-8297.
Kim, Y.J., Kim, K.P., Rhee, H. J., Das, S. Rafter, J.D., Oh, Y.S., and Cho, W. (2002) “Internalized Secretory Phospholipase A2 Acts on the Perinuclear Membranes” J. Biol. Chem. 277, 9358-65.
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Kulkarni, S., Das, S., Funk, C.D., Murray, D., and Cho, W. (2002) “A molecular basis of specific subcellular localization of the C2-like domain of 5-lipoxygenase” J. Biol. Chem. 277, 13167-13174.
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Gesquire, L., Cho, W., and Subbaiah, P.V. (2002) “Roles of group IIa and group V secretory phospholipases A2 in the metabolism of lipoproteins. Substrate specificities of the enzymes, and the regulation of their activities by sphingomyelin,” Biochemistry 41, 4911-4920.
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Muñoz, N.M., Boetticher, E., Sperling, A.I., Kim, K.P., Meliton, A., Lambertino, A., Cho, W., and Leff, A.R. (2002) “Deveolpment of highly sensitive sandwich enzyme-linked immunosorbent assay (sELISA) for group V phospholipase A2 (gVPLA2): determination of gVPLA2 in human epithelial cells and eosinophils” J. Immunol. Methods, 262, 41-51.
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Das, S. and Cho, W. (2002) “Roles of Catalytic Domain Residues in Interfacial Binding and Activation of Group IV Cytosolic Phospholipase A2,” J. Biol. Chem. 277, 23838–23846.
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Stahelin, R. V. Long, F., Diraviyam, K., Bruzik, K.S., Murray, D. and Cho, W. (2002) “Phosphatidylinositol-3-Phosphate Induces the Membrane Penetration of the FYVE Domains of Vps27p and Hrs” J. Biol. Chem. 277, 26379-26388.
Kim, Y.J., Kim, K.P., Han, S.K., Munoz, N.M., Zhu, X., Sano, H, Leff, A.R., and Cho, W. (2002) “Group V Phospholipase A2 Induces Leukotriene Biosynthesis in Human Neutrophils through the Activation of Group IVA Phospholipase A2″ J. Biol. Chem. 277, 36479-36488
Karathanassis, D., Stahelin, R.V., Bravo, J., Perisic, O., Pacold, C. M., Cho, W., Williams, R.L. (2002) “Binding of the PX domain of p47phox to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction” EMBO J. 21, 5057-5068
2001
Bittova, L., Stahelin, R. V. and Cho, W. (2001) “Roles of Ionic Residues of the C1 Domain in Protein Kinase C-α Activation and the Origin of Phosphatidylserine Specificity,” J. Biol. Chem. 276, 4218-4226.
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Myou, S., Sano, H., Fujimura, M., Zhu, X., Kurashima, K., Kita, T., Nakao, S., Nonomura, A., Takanobu, S., Kim, K.P., Muñoz, N. M., Cho, W., and Leff, A. R. (2001) “Inhibition of cytosolic PLA2 and lysophospholipid synthesis blocks antigen-induced eosinophil migration and airway hyper responsiveness,” Nature Immunol 2, 145-149
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Kim, K.-P., Rafter, J. D., Bittova, L., Han, S.-K., Snitko, Y., Muñoz, N.M., Leff, A. R., and Cho, W (2001) “Mechanism of Human Group V Phospholipase A2-Induced Leukotriene Biosynthesis in Human Neutrophils: A Potential Role of Heparan Sulfate Binding in PLA2 Internalization and Degradation,” J. Biol. Chem. 276, 11126–11134.
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KomStahelin, R. V. and Cho, W. (2001) “Roles of ionic, aliphatic, and aromatic residues in membrane-protein interactions: A surface plasmon resonance study on phospholipases A2,” Biochemistry 40, 4672-4678
Cho, W. (2001) “Membrane targeting by C1 and C2 domains,” J. Biol. Chem. (minireview) 276, 32407-32410.
Cho, W., Bittova, L., and Stahelin, R. V. (2001) “In vitro Membrane Binding Assays for Peripheral Proteins” Anal. Biochem. (review article) 296, 153-161.
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Zhu, X., Sano, H., Kim, K.P., Sano, A., Boetticher, E., Muñoz, N.M., Cho, W., and Leff, A.R. (2001) “Role of MAP Kinase-Mediated Cytosolic Phospholipase A2 Activation in Arachidonic Acid Metabolism in Human Eosinophils” J. Immunol 167, 461-468
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Stahelin, R. V. and Cho, W. (2001) “Roles of Calcium Ions in Membrane Binding and Subcellular Targeting of C2 Domains” Biochem. J. 359, 679-685
2000
Muñoz, N. M., Kim, K., Han, S.-K., Boetticher, E. Sperling, A. I. Sano, H. Zhu, X. Cho, W., and Leff, A. R. (2000) “Characterization of Monoclonal Antibodies Specific for 14 kDa human group V Secretory Phospholipase A2,” Hybridoma 19, 171-176
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Kim, K., Han, S.-K., Hong, M., and Cho, W (2000) “Molecular Basis for Phosphatidylcholine Preference of Human Group V Phospholipase A2,” Biochem. J. 348, 643-647
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Bitto, E., Li, M., Tikhonov, A., Schlossman, M. L., Cho, W. (2000) “Mechanism of vesicle aggregation by annexin I,” Biochemistry 39, 13469-13477
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Cho, W. (2000) “Structure and function of group V phospholipase A2,” Biochim. Biophys. Acta (review article) 1488, 48-58.
2016
Cho, W. Kim, H., Hu, Y. (2016) High-throughput fluorometric assay for membrane-protein interaction Methods in Mol. Biol. 1376, 163-174
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Park, M-J, Sheng, R., Silkov, A., Jung, D-J, Wang, Z-G, Xin, Y., Kim, H., Thiagarajan-Rosenkranz, P., Song, S., Yoon, Y., Nam, W., Kim, I., Kim, E., Lee, D-G, Chen, Y., Singaram, I., Wang. L. Jang, M.H., Hwang, C.-S., Honig, B., Ryu, S.H., Lorieau, J., Kim, Y-M, and Cho, W. (2016) SH2 domains serve as lipid binding modules for pTyr-signaling proteins Mol. Cell 62, 7-20. [Highlighted in Nature Reviews in Molecular Cell Biology (May, 2016) and Cancer Discovery; Recommended in Faculty of 1000 Prime being of special significance (Prywes R: F1000Prime Recommendation of Evaluation [Park MJ et al., Mol Cell 2016, 62(1):7-20]. In F1000Prime, 14 Apr 2016; DOI: 10.3410/f.726271123.793516567. F1000Prime.com/726271123#eval793516567)]
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Francis, K.R., Ton, A.N., Xin, Y., O’Halloran, P.E., Wassif, C.A., Malik, N., Williams, I.M., Cluzeau, C.V., Trivedi, N.S., Pavan, W.J., Cho, W., Westphal, H., Porter, F.D. (2016) Modeling the cholesterol synthesis disorder Smith-Lemli-Opitz Syndrome with iPS cells reveals Wnt/b-catenin defects are causative for neural phenotype. Nature Medicine 22, 388-396 [Featured in News & Views]
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Komura, N., Suzuki, K. G. N., Ando, H., Konishi, M., Koikeda, M., Imamura, A., Chadda, R., Fujiwara, T.K., Tsuboi, H., Sheng, R., Cho, W., Ishida, H., Kusumi, A., Kiso, M. (2016) Raft-based interactions of gangliosides with a GPI-anchored receptor. Nature Chem. Biol. 12, 402-410
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Sheng, R., Jung, D-J, Silkov, A., Kim, H., Singaram, I., Wang, Z-G, Xin, Y., Kim, E., Park, M-J, Rosenkranz, P.T., Smrt, S., Honig, B., Baek, K., Ryu, S.H., Lorieau, J., Kim, Y-M, and Cho, W. (2016) Lipids regulate Lck activity through their interactions with the Lck SH2 domain. J. Biol. Chem.291, 17639-50
2013
Jung, H.-R., Cho, W., Choi, J.-C., Doh, J. (2013) Microfabricated platforms to modulate and monitor T cell synapse assembly, WIREs Nanomedicine & Nanobiotechnology
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Tsukamoto, K., Wales, T. E., Daniels, K., Pal, R., Sheng, R., Cho, W., Stafford, W., Engen, J. R., Krieger, M., and Kocher, O. (2013) Non-Canonical Role of the PDZ4 domain of the Adaptor Protein PDZK1 in the Regulation of the Hepatic HDL receptor SR-BI. J. Biol. Chem. 288,19845-19860
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Francis, K.R., Ton, A.N., Xin, Y., O’Halloran, P.E., Wassif, C.A., Malik, N., Williams, I.M., Cluzeau, C.V., Trivedi, N.S., Pavan, W.J., Cho, W., Westphal, H., Porter, F.D. (2016) Modeling the cholesterol synthesis disorder Smith-Lemli-Opitz Syndrome with iPS cells reveals Wnt/b-catenin defects are causative for neural phenotype. Nature Medicine 22, 388-396 [Featured in News & Views]
Kim, H., Afsari, H. S., and Cho, W. (2013) High-Throughput Fluorescence Assay for Membrane-Protein Interaction J. Lip. Res. 54, 3531-3538
2011
J. K. Bolger, W. Tian, W. R. Wolter, W. Cho, M. A. Suckow and M. J. Miller (2011) Synthesis and evaluation of 5-lipoxygenase translocation inhibitors from acylnitroso hetero-Diels Alder cycloadducts Org Biomol Chem. 9, 2999-3010
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Morales KA, Lasagna M, AV, Yoon Y, Reinhart GD, Lee JC, Cho W, Li P, Igumenova TI (2011) Pb>2+ as modulator of protein-membrane interactions J Am Chem Soc.133,10599-10611 PMID: 21615172
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Long, F. Cho, w., and Ishii, Y. (2011) Expression and purification of 15N- and 13C-isotope labeled 40-residue human Alzheimer’s peptide for NMR-based structural analysis, Prot. Purif. 9,16-24 PMID: 21640828
Silkov, A., Yoon, Y., Lee, H., Gokhale, N., Stahelin, R.V., and Cho, W* and Murray, D.* (2011) Genome-Wide Identification of Lipid Binding Domains and Prediction of Their Membrane Binding Properties: An ANTH/ENTH domain Study J. Biol. Chem 286, 34155-34163 (*co-corresponding authors)
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Yoon, Y., Lee, P.J., Kurilova, S., and Cho, W. (2011), In Situ Quantitative Imaging of Cellular Lipids Using Molecular Sensors Nature Chemistry 3, 868-74. PMID: 22024883 [Featured in ASBMB Today (New protein sensors to quantify phosphoinositides in situ), RSC Chemistry World (New probe throws light on cellular lipids), ScienceDaily (New Membrane Lipid Measuring Technique May Help Fight Disease) and UIC News]
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Lucas, N. and Cho, W., (2011) Phosphatidylserine Binding Is Essential for Plasma Membrane Recruitment and Signaling Function of PDK1 J. Biol. Chem. 286, 41265-72
McCary, CA, Yoon, Y., Panagabko, C, Cho, W., Atkinson, J. and Cook-Mills, J., (2011) Vitamin E isoforms directly bind PKC and differentially regulate activation of PKC Biochem. J. 441,189-198
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Rowland, M., Bostic, H., Gong, D., Spears, A., Lucas, N., Cho, W., Cravatt, B., and Best, M. (2011) Phosphatidylinositol (3,4,5)-trisphosphate activity probes for the labeling and proteomic characterization of protein binding partners Biochemistry. 50, 11143-61. PMID: 22074223
Chen, H., Málková, Š., Cho, W, and Schlossman, M.L. (2011) Configuration of membrane-bound proteins by x-ray reflectivity J. Appl. Phys. 110, 102215
2009
Denghuang Gong, Matthew D. Smith, Debasis Manna, Heidi Bostic, Wonhwa Cho and Michael D. Best (2009) Microplate-Based Characterization of Protein-Phosphoinositide Binding Interactions Using Synthetic Biotinylated Head Group Analogues, Bioconj. Chem 20, 310-6.
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Chen W, Goldfine H, Ananthanarayanan B, Cho W, Roberts MF. (2009) Listeria monocytogenes Phosphatidylinositol-Specific Phospholipase C: Kinetic Activation and Homing in on Different Interfaces (dagger), Biochemistry, 48, 3578-92.
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Francis, K.R., Ton, A.N., Xin, Y., O’Halloran, P.E., Wassif, C.A., Malik, N., Williams, I.M., Cluzeau, C.V., Trivedi, N.S., Pavan, W.J., Cho, W., Westphal, H., Porter, F.D. (2016) Modeling the cholesterol synthesis disorder Smith-Lemli-Opitz Syndrome with iPS cells reveals Wnt/b-catenin defects are causative for neural phenotype. Nature Medicine 22, 388-396 [Featured in News & Views]
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Meerscharet, K., Tun, M.P., Remue, E., De Ganck, A., Houthuys, E., Boucherie, C., Vanloo, B., Degeest, G., Vandekerckhove, J., Zimmermann, P., Bhardwaj, N., Lu, H., Cho, W., Gettemans, J. (2009), The PDZ domain of Zona Occuldens-1 and -2 is a phosphoinositide-binding domain. Cell. Mol. Life Sci.. 66:3951–3966 (*co-corresponding authors).
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Chen, C.-H., Malkova, S., Long, F., Garde, S., Cho, W., and Schlossman, M. L.. (2009) Configuration of PKCa-C2 Domain Bound to SOPC:SOPS Lipid Monolayers, Biophys. J. 97, 2794 – 2802
Lee, J.S., Kim, I.S., Kim, J.H., Cho, W., Suh, P.G., Ryu, S.H. (2009) Determination of EGFR endocytosis kinetic by auto-regulatory association of PLD1 with mu2. PLoS One. 4(9):e7090
2007
Kwatia, M.A, Doyle, C.B, Cho, W., Enhorning, G., and Ackerman, S.J (2007) “The Combined Activities of Secretory Phospholipases and Eosinophil Lysophospholipases Induce Pulmonary Surfactant Dysfunction by Phospholipid Hydrolysis,” J. Allergy Clin Immunol 119, 838-847.
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Chaudhari, A., Mahfouz, M., Fialho, A.M., Yamada, T., Granja, A.T., Zhu, Y., Hashimoto, W., Schlarb-Ridley, B., Cho, W., Gupta, T.D., and Chakrabarty, A.M. (2007) “Cupredoxin – Cancer Interrelationship: Azurin Binding with EphB2, Interference in EphB2 Tyrosine Phosphorylation, and Inhibition of Cancer Growth” Biochemistry 46, 1799-1810
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Melowic, H.R., Stahelin, R.V., Blatner, N.R., Tian, W., Hayashi, K., Altman, A., and Cho, W., (2007) “Mechanism of Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cθ” J. Biol. Chem. 282, 21467-76
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Lamour, N.F., Stahelin, R.V., Wijesinghe, D.S., Maceyka, M., Wang, E., Allegood, J.C., Cho, W., Merrill, Jr., A.H., and Chalfant, C.E. (2007) “Ceramide kinase utilizes ceramide provided by ceramide transport protein. Localization to subcellular compartments of eicosanoid synthesis” J. Lipid Res. 48, 1293-304
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Stahelin, R.V., Karathanassis, D.,Murray, D., Williams, R.L., Cho, W. (2007) “Structural and Membrane Binding Analysis of the PX Domain of Bem1p: Basis of Phosphatidylinositol-4-Phosphate Specificity” J. Biol. Chem. 282, 25737-25747
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Blatner, N.R., Wilson, M.I. Lei, C., Hong, W., Murray, D., Williams, R.L. and Cho, W. (2007) “The Structural Basis of Novel Endosome Anchoring Activity of KIF16B Kinesin,” EMBO J. 26, 3709-3719
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Stahelin, R.V., Subramanian, P., Vora, M., Cho, W., and Chalfant. C.E., (2007) “Ceramide-1-phosphate binds group IVA cytosolic phospholipase A2 via a novel site in the C2 domain,” J. Biol. Chem. 282, 20467-74
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Muñoz, N.M., Meliton, A.Y., Arm, J.P., Zhu, X., Bonventre, J.V., Cho, W., and Leff. A.R. (2007) “Deletion of Secretory Group V Phospholipase A2 Attenuates Cell Migration and Airway Hyperresponsiveness in Immune-Sensitized Mice,” J. Immunol. 179, 4800-4807
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Hom, R., Vora, M., Regner, M., Subach, O.M., Cho, W., Verkhusha, V.V., Stahelin R.V., and Kutateladze, T.G. (2007) “pH-dependent binding of the EHTH domain of epsin and the ANTH domain of AP180 to PI(4,5)P2-containing bilayers” J. Mol. Biol. 373, 412-23
Manna, D., Albanese, A., Park, W.S., and Cho, W. (2007) “Mechanistic Basis of Differential Cellular Responses of PtdIns(3,4)P2- and PtdIns(3,4,5)P3-Binding PH Domains,” J. Biol. Chem. 282, 32093-32105
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Bhardwaj, N., Stahelin, R.V., Cho, W., Lu, H. (2007) “MeTaDoR: A comprehensive resource for membrane targeting domains and their host proteins,” Bioinformatics 23, 3110-3112
Manna, D. and Cho, W. (2007) “Real-Time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids,” Methods Enzymol. 434, 15-27
2005
Cho, W. and Stahelin, R.V. “Membrane-protein interactions in cell signaling and membrane trafficking,” (2005) Ann. Rev. Biophys. Biomol. Struct. 34, 119-151
Taha, T.A., Kitatani, K., Bielawski, J., Cho, W., Hannun, Y. A., and Obeid, L.M. (2005) “TNF induces the loss of sphingosine kinase-1 by a cathepsin B dependent mechanism,” J. Biol. Chem. 280, 17196-17202
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Subramanian, P., Stahelin, R.V., Szulc, Z., Bielawska, A., Cho, W. and Chalfant, C.E. (2005) “Ceramide-1-phosphate acts as a positive allosteric activator of cytosolic phospholipase A2 and enhances the interaction of the enzyme with phosphatidylcholine” J. Biol. Chem. 280, 17601-17607
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Stahelin, R.V. Digman, M.A., Medkova, M., Ananthanarayanan, B., Rafter, J.D., Melowic, H. and Cho, W. (2005) “Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cε: MECHANISTIC DIFFERENCES BETWEEN PKCδ and ε” J. Biol. Chem. 280, 19784-19793
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Angulo, Y, Gutiérrez, J.M., Soares, A.M., Cho, W., and Lomonte, B. (2005) “Myotoxic and cytolytic activities of dimeric Lys49 phospholipase A2 homologues are reduced, but not abolished, by a pH-induced dissociation,” Toxicon, 46(3), 291-6
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Cho, W. and Stahelin, R.V. “In vitro and cellular membrane binding mechanisms of membrane targeting domains,” (2005) In “Protein-Lipid Interactions”, Tamm, L. K. Ed., Academic Press, N.Y. pp. 369-401
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Malkova, S., Long, F., Stahelin, R.V. Pingali, S.V., Murray, D., Cho, W., and Schlossman, M. (2005) “Determination of the depth of membrane insertion of C2 domains by X-ray reflectivity measurements” Biophys. J. 89, 1861-1873
Stahelin, R.V., Wang, J., Blatner, N.R. Rafter, J.D., Murray, D., and Cho, W. (2005) “The origin of C1A-C2 interdomain interactions in protein kinase Cα” J. Biol. Chem. 280, 36452-36463
Gokhale, N. A. Abraham, A. Digman, M.A., Gratton, E., and Cho, W. (2005) “Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 tetramer” J. Biol. Chem. 280, 42831-42840
Stahelin, R.V., Hwang, J.H., Kim, J.-H., Park, Z.-Y., Johnson, K.R., Obeid, L.M., and Cho, W. (2005) “The mechanism of membrane targeting of human sphingosine kinase 1,” J. Biol. Chem. 280, 43030-43038
2003
Lee, B.-I., Mustafi, D., Cho, W., and Nakagawa, Y. (2003) “Characterization of Calcium Binding Properties of Lithostathine,” J. Biol. Inorg. Chem. 8, 341-347
Stahelin, R.V. Forslund, R.E., Wink, D.J. and Cho, W. (2003) “Development of a biochemistry laboratory course with a project-oriented goal” Biochemistry and Molecular Biology Education. 31, 106-112
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Stahelin, R.V., Rafter, J.D., Das, S. and Cho, W. (2003) “A Molecular Basis of Differential Subcellular Localization of C2 Domains of Protein Kinase C-α and Group IVa Cytosolic Phospholipase A2,” J. Biol. Chem. 278, 12452-12460
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Stahelin, R.V. Burian, A., Bruzik, K.S., Murray, D. and Cho, W. (2003) “Membrane binding mechanisms of the PX domains of NADPH oxidase p40phox and p47phox,” J. Biol. Chem. 278, 14469-14479
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Diraviyam, K., Stahelin, R. V., Cho, W., and Murray, D. (2003) “Computer modeling of the membrane interaction of FYVE domains,” J. Mol. Biol. 328, 721-736
Liu, Y., Bruzik, K.S., Ananthanarayanan, B., and Cho, W. (2003) “New aspects of formation of 1,2-cyclic phosphate by phospholipase C-d1,” Bioorg. Med. Chem. Lett. 11, 2471-2475
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Cho, W., Digman, M.A., Ananthanarayanan, B. and Stahelin, R.V. (2003) “Bacterial expression and purification of C1 and C2 domains of protein kinase C isoforms” in Methods in Molecular Biology, vol. 233: Protein Kinase C Protocols, Ed., Newton, A.C., K. Humana Press, Totowa, New Jersey, 291-298.
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Das, S., Dixon, J.E. and Cho, W. (2003) “Mechanism of membrane targeting and activation of PTEN,” Proc. Natl. Acad. Sci. USA 100, 7491-7496.
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Stahelin, R.V., Long, F., Murray, D. Peter, B.J., De Camilli, P., McMahon, H.T., and Cho, W. (2003) “Contrasting membrane binding mechanisms of ENTH and ANTH domains,” J. Biol. Chem. 278, 28993-28999
Muñoz, N.M., Kim, Y.J., Kim, K.-P., Zhu, X., Leff, A.R., and Cho, W (2003) “Group V Phospholipase A2 Induces Group IVA Phospholipase A2-Independent Cysteinyl Leukotriene Biosynthesis in Human Eosinophils,” J. Biol. Chem. 278, 38813-38820
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Murakami, M, Das, S., Kim, Y.J., Cho, W., Kudo, I. (2003), “Perinuclear localization of cytosolic phospholipase A2α is crucial but notobligatory for coupling with cyclooxygenases” FEBS Lett 546, 251-256
Wehbi, H., Feng, J., Kolbeck, J., Ananthanarayanan, B., Cho, W., and Roberts, M. F. (2003) “Investigating the Interfacial Binding of Bacterial Phosphatidylinositol-Specific Phospholipase C” Biochemistry 42, 9374-9382
Das, S., Rafter, J.D., Kim, K.-P., Gigy, S.P., and Cho, W. (2003) “Mechanism of group IVA cytosolic phospholipase A2 activation by phosphorylation,” J. Biol. Chem. 278, 41431-41442.
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Ananthanarayanan, B., Stahelin, R. V., Digman, M. A., and Cho, W. (2003) “Activation Mechanisms of Protein Kinase C Isoforms Are Determined by the Ligand Affinity and Conformational Flexibility of Their C1 Domains,” J. Biol. Chem. 278, 46886-46894.
1990s
Lomonte, B. Angulo, Y. Rufini, S. Cho, W. Giglio, J.R. Ohno, M. Daniele, J.J, Geoghegan, P. and Gutiérrez, J.M. (1999) “Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro” Toxicon 37(1):145-58
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Cho, W., Han, S.-K., Lee, B.-I., Snitko, Y. and Dua, R. (1999) “Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2” in Methods in Molecular Biology, vol. 109: Lipase and Phospholipase Protocols, Eds, Doolittle, M.H. and Reue, K. Humana Press, Totowa, New Jersey pp 31-38
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Cho, W. and Shen, Z. (1999) “Efficient Immobilization of Phospholipase A2” in Methods in Molecular Biology, vol. 109: Lipase and Phospholipase Protocols, Eds, Doolittle, M.H. and Reue, K. Humana Press, Totowa, New Jersey pp. 303-307
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Cho, W., Wu, S.-K., Yoon, E.T. and Lichtenbergova, L. (1999) “Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates” in Methods in Molecular Biology, vol. 109: Lipase and Phospholipase Protocols, Eds, Doolittle, M.H. and Reue, K. Humana Press, Totowa, New Jersey pp.7-17
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Bittova, L., Sumandea, P.M. and Cho, W. (1999) “A Structure-Function Study of C2 Domain of Cytosolic Phospholipase A2: Identification of essential calcium ligands and hydrophobic membrane binding residues” J. Biol. Chem. 274, 9665-9672
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Han, S.-K., Kim, K.P., Koduri, R., Bittova, L., Munoz, N.M., Leff, A. R., Wilton, D.C., Gelb, M.H., and Cho, W. (1999) “Role of Trp31 in high membrane binding and pro-inflammatory activity of human group V phospholipase A2” J. Biol. Chem. 274, 11881-11888
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Wilson, H.A., Waldrip, J.B., Nielson, K.H., Judd, A.M., Han, S.-K., Cho, W., Sims, P.J., and Bell, J.D. (1999) “Mechanisms by which elevated intracellular calcium induces S49 cell membranes to become susceptible to the action of secretory phospholipase A2” J. Biol. Chem. 274, 11494-11504
Medkova, M. and Cho, W. (1999) “Interplay of the C1 and C2 Domains of Protein Kinase C-α in Its Membrane Binding and Activation” J. Biol. Chem 274, 19852-19861
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Snitko, Y., Han, S.-K. and Cho, W. (1999) “Interfacial and Substrate Binding of Human Pancreatic Phospholipase A2: A Comparison among Mammalian Pancreatic Phospholipases A2” Biochemistry 38, 7803-7810
Lee, B.-I., Dua, R. and Cho, W. (1999) “A Structural Determinant of the Unique Interfacial Binding Mode of Bovine Pancreatic Phospholipase A2” Biochemistry 38, 7811-7818
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Gelb, M.H., Cho, W. and Wilton, D.C. (1999) “Interfacial Binding of Secreted Phospholipases A2: More Than Electrostatics and a Major Role for Tryptophan” Current Opinions in Structural Biology 9, 428-432
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Zhu, X., Muñoz, N.M., Kim, K.-P., Sano, H., Cho, W, and Leff, A. R., (1999) “Cytosolic Phospholipase A2 Activation is Essential for β1 and β2 Integrin-Dependent Adhesion to Human Eosinophils,” J. Immunol 163, 3423-3429
Bitto, E. and Cho, W. (1999) “A Structural Determinant of the Vesicle Aggregation Activity of Annexin I,” Biochemistry 38, 14094-14100
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Sumandea, M., Das, S., Sumandea, C. and Cho, W. (1999) “Roles of Aromatic Residues in High Interfacial Activity of Naja naja atra Phospholipase A2,” Biochemistry 38, 16290-16297
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Han, S.-K., Yoon, E.T. and Cho, W. (1998) “Functional Expression and Characterization of Human Secretory Class V Phospholipase A2” Biochem. J. (Communication) 331, 353-357
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Medkova, M. and Cho, W. (1998) “Differential membrane-binding and activation mechanisms of Protein Kinase C-α and -ε” Biochemistry 37, 4892-4900
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Ghosh, A.K., Kinkaid, J.F., Cho, W., Walters, D.E., Krishnan, K., Hussain, K.A., Koo, Y., Cho, H., Rudall, C., Holland, L., Buthod (1998) “Potent HIV Protease Inhibitors Incorporating High Affinity P2-Ligands and (R)-(Hydroxyethylamino)-Sulfonamide Isostere” Bioorg. Med. Chem. Lett. 8, 687-690
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Ghosh, A. K., K. Krishnan, D. E. Walters, W. Cho, Y. Koo, H. Cho, L. Holland, J. Buthod (1998) “Structure Based Design: Novel Spirocyclic Ethers as Nonpeptidal P2-Ligands for HIV Protease Inhibitors” Bioorg. Med. Chem. Lett. 8, 979-982
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Medkova, M. and Cho, W. (1998) “Mutagenesis of the C2 Domain of Protein Kinase C-α: Differential Roles of Ca2+ Ligands and Membrane Binding Residues” J. Biol. Chem. 273, 17544-17552
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Bitto, E. and Cho, W. (1998) “Roles of Individual Domains of Annexin I in Its Vesicle Binding and Vesicle Aggregation: A Comprehensive Mutagenesis Study” Biochemistry 37, 10231-10237
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Lichtenbergova, L., Yoon, E. T. and Cho, W. (1998) “Membrane Penetration of Cytosolic Phospholipase A2 is Necessary for Its Interfacial Catalysis and Arachidonate Specificity” Biochemistry 37, 14128-14136
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Lin, Y. Ghomashchi, F., Nielsen, R., Snitko, Y., Yu, B.-Z., Han, S.-K., Cho, W., Wilton, D. C., Jain, M.K., Robinson, B.H. and Gelb, M. H. (1998) “Binding of bee venom and human group IIa phospholipases A2 to membranes: a minor role for electrostatics.” Biochem Soc Trans. 26, 341-5
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Koduri, R., Baker, S.F., Snitko, Y., Han, S.-K., Cho, W., Wilton, D. C. and Gelb, M. H. (1998) “Action of Human Group IIa Secreted Phospholipase A2 on Cell Membranes: Vesicle but not Heparanoid Binding Determines Rate of Fatty Acid Release” J. Biol. Chem. 273, 32142-32153
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Snitko, Y., Yoon, E. T. and Cho, W. (1997) “High Specificity of Human Secretory Class II Phospholipase A2 for Phosphatidic Acid” Biochem. J. 321, 737-741
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Han, S.-K., Yoon, E. T., Scott, D.L., Sigler, P.B. and Cho, W. (1997) “Structural Aspects of Interfacial Adsorption: A Crystallographic and Site-directed Mutagenesis Study of the Phospholipase A2 from the Venom of Agkistrodon piscivorus piscivorus” J. Biol. Chem. 272, 3573-3582
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Han, S.-K., Lee, B.-I., and Cho, W. (1997) “The Bacterial Expression and Kinetic Characterization of Human Pancreatic Phospholipase A2” Biochim. Biophys. Acta 1346, 185-192
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Kim, Y., Lichtenbergova, L., Snitko, Y., and Cho, W. (1997) “A Phospholipase A2 Kinetic and Binding Assay Using Phospholipid-Coated Hydrophobic Beads” Anal. Biochem. 250, 109-116
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Snitko, Y., Koduri, R., Han, S.-K., Othman, R., Baker, S.F., Molini, B. J., Wilton, D. C., Gelb, M. H., and Cho, W. (1997) “Mapping the Interfacial Binding Surface of Human Secretory Class II Phospholipase A2” Biochemistry 36, 14325-14333
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Mukhopadhyay, S. and Cho, W. (1996) “Interactions of Annexin V with Phospholipid Monolayers“ Biochim. Biophys. Acta 1276, 58-62
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Lee, B.-I., Yoon, E.T. and Cho, W. (1996) “Roles of Surface Hydrophobic Residues in the Interfacial Binding of Bovine Pancreatic Phospholipase A2” Biochemistry 35, 4231-4240
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Dua, R., Wu, S.-K., and Cho, W. (1995) “A Structure-Function Study of Bovine Pancreatic Phospholipase A2 Using Polymerized Mixed Liposomes” J. Biol. Chem. 270, 263-268
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Shen, Z. and Cho, W. (1995) “Highly Efficient Site-Specific Immobilization of Phospholipases A2” J. Lipid Res. 36, 1147-1151
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Shen, Z. and Cho, W. (1995) “Membrane Leakage Induced by Synergetic Action of Lys-49 and Asp-49 Agkistrodon Piscivorus Piscivorus Phospholipases A2: Implications in Their Pharmacological Activities” Int. J. Biochem. Cell Biol. 27/10, 1009-1013
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Dua, R. and Cho, W. (1994) “Inhibition of Human Secretory Class II Phospholipase A2 by Heparin” Eur. J. Biochem. 221, 481-490
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Wu, S.-K. and Cho, W. (1994) “A continuous Fluorometric Assay for Phospholipases Using Polymerized Mixed Liposomes” Anal. Biochem. 221, 152-159
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Shen, Z., Wu, S.-K. and Cho, W. (1994) “Effects of Specific Fatty-Acid Acylation of Phospholipase A2 on Its Interfacial Binding and Catalysis” Biochemistry 33, 11598-11607
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Wu, S.-K. and Cho, W. (1993) “Use of Polymerized Liposomes to Study Interactions of Phospholipase A2 with Biological Membranes” Biochemistry 32, 13902-13908
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Cho, W. and Kézdy, F. J. (1991) “Chromogenic Phospholipase A2 Substrates and Assays” Methods Enzymol. 197, 75-78
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Cho, W., Markowitz, M.A., and Kézdy, F.J. (1988) “A New Class of Phospholipase A2 Substrates: Kinetics of the Phospholipase A2-Catalyzed Hydrolysis of 3-Acyloxy-4-Nitrobenzoic Acids” J. Am. Chem. Soc. 110, 5166-5171
Cho, W., Tomasseli, A.G., Heinrikson, R.L., and Kézdy, F.J. (1988) “The Chemical Basis for Interfacial Activation of Monomeric Phospholipase A2” J. Biol. Chem. 263, 11237-11241
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Suh, J., Cho, W., and Chung, S. (1985) “Carboxypeptidase A-Catalyzed Hydrolysis of α-(Acylamino)-cinnamoyl Derivatives of L-β-Phenyllactate and L-Phenylalanate: Evidence for Acyl-Enzyme Intermediates” J. Am. Chem. Soc. 107, 4530-4535
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Maraganore, J.M., Merutka, G., Cho, W., Welches, W., Kézdy, F.J., and Heinrikson, R.L. (1984) “A New Class of Phospholipase A2 with Lysine in Place of Aspartate 49: Functional Consequences of Calcium and Substrate Binding” J. Biol. Chem. 259, 13839-13843